Insertion junction: LMJ.RY0402.212437_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.212437
Chromosome:chromosome 9
Location:751485
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g402450 (1 of 1) 3.6.4.8 - Proteasome ATPase / RP triple-A protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTCCGCAACACCAGTGACCGCCCCGAG
Internal bar code:CAATGGAAGGCAGGGTACGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:932
LEAP-Seq percent confirming:99.4813
LEAP-Seq n confirming:8438
LEAP-Seq n nonconfirming:44
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GTCAAGAACGCTAGTTCGCC
Suggested primer 2:GAAGCGACTCATCTTCCAGG