| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212461 |
| Chromosome: | chromosome 5 |
| Location: | 1577645 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243452 | (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTCCCTCTTGCCAAGTACATACCACATA |
| Internal bar code: | GATTGACGTGGTTAGGCGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 742 |
| LEAP-Seq percent confirming: | 99.6219 |
| LEAP-Seq n confirming: | 12646 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTTGCTCCGAGCTGATAG |
| Suggested primer 2: | AGAGTGATTGATGTGCTGCG |