| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212492 |
| Chromosome: | chromosome_13 |
| Location: | 2802057 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre13.g583000 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AGGCGCAAGGACATTGCCGGCCTCTGCGTT |
| Internal bar code: | GGGAACAACCAGCGTTGCGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1043 |
| LEAP-Seq percent confirming: | 95.8949 |
| LEAP-Seq n confirming: | 36044 |
| LEAP-Seq n nonconfirming: | 1543 |
| LEAP-Seq n unique pos: | 107 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACGGTTGCTGTTTGGCT |
| Suggested primer 2: | GGTAGCGAGCAAAGAACGTC |