Insertion junction: LMJ.RY0402.212507_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.212507
Chromosome:chromosome 6
Location:8562347
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g308400 PTN1 (1 of 1) 3.1.3.16//3.1.3.48//3.1.3.67 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase // Protein-tyrosine-phosphatase / PTPase // Phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase / Phosphatidylinositol-3,4,5-trisphosphate 3-phosphohydrolase; PTEN PI-3 phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCTGCTGCTGCAGCTGCTGGGAGAGGTC
Internal bar code:CGTAAGCGACATGTCGGCCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:78.7529
LEAP-Seq n confirming:1023
LEAP-Seq n nonconfirming:276
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GTAGAGAGGCTGACCCGTTG
Suggested primer 2:GCTCCCCTTCACTCTAGGCT