Insertion junction: LMJ.RY0402.212520_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.212520
Chromosome:chromosome 6
Location:5329146
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g283250 POB9 Proteome of basal body 9 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTCGAGACCACCAGACATATACACACACA
Internal bar code:GCAATCGCAACTTGGAGCTCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:52
LEAP-Seq percent confirming:21.0084
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:376
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCACACCAACTCCTACA
Suggested primer 2:GCGGACTTCTTCTGTTTTCG