| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212566 |
| Chromosome: | chromosome 12 |
| Location: | 8449317 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548350 | (1 of 16) IPR011044 - Quinoprotein amine dehydrogenase, beta chain-like | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCACAGGCGCATGCACAGGGGCACACAAG |
| Internal bar code: | AGATATCTAGTATCTTCGTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1013 |
| LEAP-Seq percent confirming: | 99.3079 |
| LEAP-Seq n confirming: | 5309 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGGTGTCCTGATTACGGGC |
| Suggested primer 2: | CCCTCCCTACTACCGGCTAC |