| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212568 |
| Chromosome: | chromosome 16 |
| Location: | 1472083 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g652750 | FMO10 | Flavin-containing monooxygenase; (1 of 8) 1.14.13.8 - Flavin-containing monooxygenase / Ziegler's enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGAGGCCCAGATGGAGTGGCGGCGCCGC |
| Internal bar code: | TAAGGGGCGGAATTCTGTTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 433 |
| LEAP-Seq percent confirming: | 99.6827 |
| LEAP-Seq n confirming: | 18535 |
| LEAP-Seq n nonconfirming: | 59 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGGTGCCAAGCACAGTC |
| Suggested primer 2: | GCCCTAACACAAAGCACCAT |