| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212574 |
| Chromosome: | chromosome 13 |
| Location: | 2058166 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g577250 | GOX14 | (1 of 20) PTHR32208:SF21 - F10A5.18; Glyoxal oxidase 14 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTCCTCCCCCGGCGTGTTCTAGCCAGCC |
| Internal bar code: | GCCTGCTACATGCGCCTAGCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 314 |
| LEAP-Seq percent confirming: | 99.8073 |
| LEAP-Seq n confirming: | 6734 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACACCTGCGTAGATGCG |
| Suggested primer 2: | GTAACATGTCACACCGTCGC |