Insertion junction: LMJ.RY0402.212581_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.212581
Chromosome:chromosome 3
Location:8196164
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g200150 (1 of 1) PTHR15139:SF0 - TUBULIN-SPECIFIC CHAPERONE C 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGCTCCGAACTTTCGAGGACAGCTGCT
Internal bar code:GCTAGTGTTGGATACTCTTTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:349
LEAP-Seq percent confirming:98.9529
LEAP-Seq n confirming:189
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GAGGCTGTAGTGGTGGTGGT
Suggested primer 2:AAATCGCGATGCAACTCTCT