| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212581 |
| Chromosome: | chromosome 3 |
| Location: | 8196166 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g200150 | (1 of 1) PTHR15139:SF0 - TUBULIN-SPECIFIC CHAPERONE C | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTGCTTCAGGCGTCGTGTAGTTCTTCC |
| Internal bar code: | TCTGCGCCGACGTATGATTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 122 |
| LEAP-Seq percent confirming: | 99.1304 |
| LEAP-Seq n confirming: | 228 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAATCGCGATGCAACTCTCT |
| Suggested primer 2: | GAGGCTGTAGTGGTGGTGGT |