| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212594 |
| Chromosome: | chromosome 1 |
| Location: | 3468981 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022000 | FIGN1,SPA1 | Putative fidgetin homolog; (1 of 1) PTHR23074//PTHR23074:SF17 - AAA ATPASE // FIDGETIN-LIKE PROTEIN 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCGTCATCTTCATAGACGAGATAGACTC |
| Internal bar code: | GGGGGGGATCGTAGGCTCCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 763 |
| LEAP-Seq percent confirming: | 97.9532 |
| LEAP-Seq n confirming: | 335 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGTATGAGGAGGCTGAG |
| Suggested primer 2: | GAATGTAGAGCTGCTTGGGC |