| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212597 |
| Chromosome: | chromosome 16 |
| Location: | 1368133 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651923 | CRI1,CRTISO1 | Carotenoid isomerase; (1 of 1) PTHR10668:SF5 - BIOGENESIS OF LYSOSOME-RELATED ORGANELLES COMPLEX 1 SUBUNIT 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGCGTGTGGCTCTGCGGCGGGGCGGGG |
| Internal bar code: | ATTTATGTAAGCTGTTCGGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 36 |
| LEAP-Seq percent confirming: | 99.5349 |
| LEAP-Seq n confirming: | 214 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGCCTCGTACTCCTCAACT |
| Suggested primer 2: | TGAGAAGATGGGAATCTGGG |