| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212626 |
| Chromosome: | chromosome 14 |
| Location: | 3036770 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628237 | CDS|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGTTGGAAGTGGGCCAGGGTGGGCAAA |
| Internal bar code: | CGGGAAGCAACAGAAGGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 524 |
| LEAP-Seq percent confirming: | 99.1701 |
| LEAP-Seq n confirming: | 717 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCCACTGTCGTACATGCC |
| Suggested primer 2: | AAAGCCCAACATGTCGAAAC |