| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212640 |
| Chromosome: | chromosome_3 |
| Location: | 478102 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g145127 | ODA11,DHC13 | Flagellar outer arm dynein heavy chain alpha | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCAGGTCGGCAGACGACACGAAGTAGAAGC |
| Internal bar code: | GGCATCTGGAACGTCGGATTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 917 |
| LEAP-Seq percent confirming: | 99.3631 |
| LEAP-Seq n confirming: | 2184 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCATAAACATACGGTCGGC |
| Suggested primer 2: | GTGAAGGACCCCAACTTCAA |