| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212640 |
| Chromosome: | chromosome_17 |
| Location: | 2671777 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre17.g717750 | TRX24 | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATTGTGTTCTTACTCATGCAGCCGCAAGCG |
| Internal bar code: | AGACTCGGAAGAGCAAGAACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1030 |
| LEAP-Seq percent confirming: | 99.4887 |
| LEAP-Seq n confirming: | 3308 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCATTTCTTTCATCCCGAG |
| Suggested primer 2: | GCGATGTGAAGATGGTGATG |