| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212660 |
| Chromosome: | chromosome 13 |
| Location: | 1750479 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g574650 | (1 of 2) 3.6.1.62 - 5'-(N(7)-methylguanosine 5'-triphospho)-[mRNA] hydrolase / M(7)GpppN-mRNA hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGCCCAGCCCCGCTTGAGCCGGCAGCGTC |
| Internal bar code: | ATCACTGGGTTACCGACCCGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 296 |
| LEAP-Seq percent confirming: | 98.5409 |
| LEAP-Seq n confirming: | 5403 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGCTGGGATTCTCTTCT |
| Suggested primer 2: | AGCTGAGGGGAAGTGAGTGA |