Insertion junction: LMJ.RY0402.212665_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.212665
Chromosome:chromosome 7
Location:2958222
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g332901 (1 of 80) IPR003882 - Pistil-specific extensin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGCTGGTGTAGGGGGATTGAAGGTTATG
Internal bar code:AGACGATGAGCACTCCTGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:902
LEAP-Seq percent confirming:99.6527
LEAP-Seq n confirming:8322
LEAP-Seq n nonconfirming:29
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:ACATGTTTTACTCGGACGCC
Suggested primer 2:CCGCCATAGTATGCCAGTTT