Insertion junction: LMJ.RY0402.212665_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.212665
Chromosome:chromosome 7
Location:2958239
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g332901 (1 of 80) IPR003882 - Pistil-specific extensin-like protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCACAGTATCTTCCCTCGCTTCCTGCAACC
Internal bar code:TTTGCAAGTGGACTCATGCCCC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:10.9375
LEAP-Seq n confirming:84
LEAP-Seq n nonconfirming:684
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACATGTTTTACTCGGACGCC
Suggested primer 2:CCGCCATAGTATGCCAGTTT