| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212673 |
| Chromosome: | chromosome 6 |
| Location: | 3605298 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278124 | (1 of 1) PTHR12894//PTHR12894:SF10 - CNH DOMAIN CONTAINING // VAM6/VPS39-LIKE PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGCAGGTCTGCAGGCGGGGGTGTATG |
| Internal bar code: | CATGACTTGACGATCGGGACCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 365 |
| LEAP-Seq percent confirming: | 94.7724 |
| LEAP-Seq n confirming: | 1541 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGGTAATCCTGTGGGG |
| Suggested primer 2: | TTGCGCTTCTTTTTCCTTGT |