| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212674 |
| Chromosome: | chromosome 6 |
| Location: | 3932273 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278186 | (1 of 1) IPR000157//IPR011251//IPR016024 - Toll/interleukin-1 receptor homology (TIR) domain // Luciferase-like domain // Armadillo-type fold | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGAGGCGTTCAAACTCGGGGCCGTGGG |
| Internal bar code: | ACCCGACTACGCTCCGTTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 326 |
| LEAP-Seq percent confirming: | 98.4848 |
| LEAP-Seq n confirming: | 1170 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGCTCCAACGATTACCG |
| Suggested primer 2: | TTGGGTTTGGGAATTAGCTG |