| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212733 |
| Chromosome: | chromosome_12 |
| Location: | 6201125 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g536600 | FAP78 | Flagellar Associated Protein | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | AAGGTGATGATCTGGCGCGGCCGCTTGGGG |
| Internal bar code: | GGGCACGACGGGGGCGACTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 137 |
| LEAP-Seq percent confirming: | 78.3019 |
| LEAP-Seq n confirming: | 166 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCTCATCATGCTAGTGAA |
| Suggested primer 2: | ACGTGCCATTCTCCAAGAAG |