| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212735 |
| Chromosome: | chromosome 12 |
| Location: | 5699206 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532650 | (1 of 1) PTHR21394:SF0 - MAU2 CHROMATID COHESION FACTOR HOMOLOG | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCATCGTTCCATGCTAGGGCCGTGCAGC |
| Internal bar code: | GTAGATCTTAACGTTCGAGCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 213 |
| LEAP-Seq percent confirming: | 94.4997 |
| LEAP-Seq n confirming: | 1426 |
| LEAP-Seq n nonconfirming: | 83 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACTTAACCAGTCACGCAC |
| Suggested primer 2: | GGGGAACGATATCTCAGCAA |