| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212736 |
| Chromosome: | chromosome 3 |
| Location: | 5553172 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g186400 | SCR1 | (1 of 3) PF03803 - Scramblase (Scramblase); Putative phospholipid scramblase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTCAGCTAGCTGCGCGCGGGAGTTTCCG |
| Internal bar code: | GTGGGATCGCACGGCTCAAGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 739 |
| LEAP-Seq percent confirming: | 95.1324 |
| LEAP-Seq n confirming: | 7759 |
| LEAP-Seq n nonconfirming: | 397 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGACTTCAACCAGCAGAACA |
| Suggested primer 2: | ACGAGTACCCGTCAATCCTG |