Insertion junction: LMJ.RY0402.212738_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.212738
Chromosome:chromosome 8
Location:4330616
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g381483 SDR15 (1 of 239) IPR016024 - Armadillo-type fold; Short-chain dehydrogenase/reductase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTCGAAACCTTATGCACCCATGCTCGTAC
Internal bar code:GTACTCCTGTACCTCGCGCCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:559
LEAP-Seq percent confirming:99.6858
LEAP-Seq n confirming:2855
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AAGTTAGAGCGACCGAACGA
Suggested primer 2:TGTCGACTGTGTGGTTTTGG