| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212759 |
| Chromosome: | chromosome 7 |
| Location: | 854730 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318551 | CYA8 | Adenylate cyclase; (1 of 1) PF00211//PF13855 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Leucine rich repeat (LRR_8) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATTATGTACTGCCTTGCTTTACCTGTCC |
| Internal bar code: | TTTAGGGACGTCTTCGTCAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 883 |
| LEAP-Seq percent confirming: | 94.4393 |
| LEAP-Seq n confirming: | 1019 |
| LEAP-Seq n nonconfirming: | 60 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTACCTCACGTTCCCATGC |
| Suggested primer 2: | CCTCCAGGCAGTAGCAGTTC |