| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212772 |
| Chromosome: | chromosome 9 |
| Location: | 6471889 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407250 | FAP270 | (1 of 3) PTHR24193//PTHR24193:SF87 - ANKYRIN REPEAT PROTEIN // SUBFAMILY NOT NAMED; Flagellar Associated Protein 270 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGGCGGTGCAGGCACTGCATACGGTGCT |
| Internal bar code: | TTCACATAGGTGCCACCTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 989 |
| LEAP-Seq percent confirming: | 99.5539 |
| LEAP-Seq n confirming: | 8258 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGATTCCTATTTCCCCG |
| Suggested primer 2: | GGTCTGGTCTGGCAGCTTAG |