Insertion junction: LMJ.RY0402.212782_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.212782
Chromosome:chromosome 10
Location:6211380
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g464400 SSA20 Predicted protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACAGAAATGTTTGCGCTCACAACAACTCA
Internal bar code:TCACCGACCCACTCGTACCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:384
LEAP-Seq percent confirming:98.9899
LEAP-Seq n confirming:294
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCTTTGCAGCTCAGTCC
Suggested primer 2:ATTGTCGTTTCCCAAATCCA