Insertion junction: LMJ.RY0402.212811_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.212811
Chromosome:chromosome 9
Location:2642434
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g390152 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTTTCTAGTTTGTGCGATGCGCATGGTT
Internal bar code:ATATTGGGGGGGGAGTGCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1004
LEAP-Seq percent confirming:99.2951
LEAP-Seq n confirming:3240
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGACATCACGTTGGAGTG
Suggested primer 2:CTTATAATCTCCGCGCAAGC