| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212814 |
| Chromosome: | chromosome 3 |
| Location: | 4000957 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171950 | PPC2,PEPC2 | (1 of 2) K01595 - phosphoenolpyruvate carboxylase (ppc); Phosphoenolpyruvate carboxylase 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCTGGGGCTACCCGAAACCCGGACGCAGA |
| Internal bar code: | CTCGCGTCCCTAAGGACCCTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 107 |
| LEAP-Seq percent confirming: | 98.8024 |
| LEAP-Seq n confirming: | 495 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCCGGTTCGTGTCTTCAC |
| Suggested primer 2: | CCTCACTTCCTGACAGAGGC |