| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212821 |
| Chromosome: | chromosome 6 |
| Location: | 5731396 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g287000 | (1 of 1) PTHR31495//PTHR31495:SF0 - FAMILY NOT NAMED // PEROXYGENASE 1-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGTGAAAGGAAGCGAAACTGGATTCATG |
| Internal bar code: | GCCCGAGTCGGGGCCCTAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 498 |
| LEAP-Seq percent confirming: | 99.6198 |
| LEAP-Seq n confirming: | 524 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGAACACGCACTAGCATTA |
| Suggested primer 2: | TCATCCACCTGTCCTTCTCC |