| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212826 |
| Chromosome: | chromosome 10 |
| Location: | 6048721 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g463200 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAACGCGGTGGTGGGCGGGATTTGGCCCC |
| Internal bar code: | CCGGCCGCCTATGGTCGGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 97.4617 |
| LEAP-Seq n confirming: | 1843 |
| LEAP-Seq n nonconfirming: | 48 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACGGACCATTGACTGTGT |
| Suggested primer 2: | CTCGTCAATAAGCAGTGGCA |