| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212833 |
| Chromosome: | scaffold_18 |
| Location: | 256266 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre18.g750047 | FMG1-A | Flagella membrane glycoprotein, minor form | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAGTAGGGTGTGTAGCTGGTGTTGCCCAC |
| Internal bar code: | ACGCGCCGCAGCAACGCAGAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 887 |
| LEAP-Seq percent confirming: | 91.5028 |
| LEAP-Seq n confirming: | 7678 |
| LEAP-Seq n nonconfirming: | 713 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGACGTTGATGGTGTTGG |
| Suggested primer 2: | TTTGATCCTTGTGTAGGGGC |