| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.212847 |
| Chromosome: | chromosome 3 |
| Location: | 5916360 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190050 | (1 of 1) PF00659 - POLO box duplicated region (POLO_box) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGGCCAGCACCACTCGCGCACTGGCAACC |
| Internal bar code: | GGAACGAATTGTACCCAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 480 |
| LEAP-Seq percent confirming: | 98.7672 |
| LEAP-Seq n confirming: | 1362 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCAGGGCTTCTATGAGTTC |
| Suggested primer 2: | CCTTCCCTCTACCATACCCC |