| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212933 |
| Chromosome: | chromosome 16 |
| Location: | 3723452 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g688950 | (1 of 8) 2.4.1.5 - Dextransucrase / Sucrose 6-glucosyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCCGACGCGTGCCCCCAGCACCCACCTG |
| Internal bar code: | GCGCACGACAGAGCGAACGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 86.3552 |
| LEAP-Seq n confirming: | 3253 |
| LEAP-Seq n nonconfirming: | 514 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGTAGTCTGTGATGGCAGC |
| Suggested primer 2: | CAGCTCGCAACTTACTTCCC |