| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.212938 |
| Chromosome: | chromosome 16 |
| Location: | 713319 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691353 | (1 of 14) PF13371 - Tetratricopeptide repeat (TPR_9) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGGCGCTGCGCGCATCGGCCACGCGGCAG |
| Internal bar code: | AGGTAGAAAGGGGATAGAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 94.8407 |
| LEAP-Seq n confirming: | 11250 |
| LEAP-Seq n nonconfirming: | 612 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATGACAGACCGCGTCTCA |
| Suggested primer 2: | TGATTTCGCATTACGCTGAG |