| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.212950 |
| Chromosome: | chromosome_1 |
| Location: | 3544423 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre01.g022750 | FAP159 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGAACCATACACGCTCCAGCTCACCAGCCG |
| Internal bar code: | CCGCTATCTATGGGAAAGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 516 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 374 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTACAAGGTGTACGGACGG |
| Suggested primer 2: | GCATGGCCAACTCTACCATT |