| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212953 |
| Chromosome: | chromosome 1 |
| Location: | 2871063 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g017400 | (1 of 33) IPR013830 - SGNH hydrolase-type esterase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGTCCTACACATGACGCGTCGGCTGCC |
| Internal bar code: | AAGCAGGGAAAGATCGACATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 99.6451 |
| LEAP-Seq n confirming: | 4212 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTAATGCTTCTGCCTG |
| Suggested primer 2: | CCTGTGATCAAGGGCAAAAT |