Insertion junction: LMJ.RY0402.212973_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.212973
Chromosome:chromosome 13
Location:3182947
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre13.g585400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCACAGCACGAAACCCTCTCTTCTCTCT
Internal bar code:TTGTCGGATCGATCTTACACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCGGTGTCAGCTCTGTAG
Suggested primer 2:TACATAATCCCCCTCCCTCC