| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.212977 |
| Chromosome: | chromosome 6 |
| Location: | 5679765 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286450 | (1 of 1) PTHR22847//PTHR22847:SF489 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCAACGTTTTCCTTTTGCTCTTTAACAC |
| Internal bar code: | TGTACTCAAGTTGAAAGATATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 84 |
| LEAP-Seq percent confirming: | 89.3939 |
| LEAP-Seq n confirming: | 118 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGAGTGAAGTTGCAGCC |
| Suggested primer 2: | CTGCGTCAAGCTTGTGTTGT |