| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213000 |
| Chromosome: | chromosome 14 |
| Location: | 181412 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g608850 | (1 of 3) PF10075 - CSN8/PSMD8/EIF3K family (CSN8_PSD8_EIF3K) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGTGGCTTTGTGGAGCGACACATCACC |
| Internal bar code: | TGCATTGGGGCCCGCCGGAAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 97.0075 |
| LEAP-Seq n confirming: | 389 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTAGAGCGCAAACTGTGGTG |
| Suggested primer 2: | GACCTCGCGAAAGTCAAGTC |