| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.213021 |
| Chromosome: | chromosome 1 |
| Location: | 6095338 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g043500 | (1 of 1) PTHR20882//PTHR20882:SF14 - FAMILY NOT NAMED // CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCGCGCCGGCCACGGCGCCCGCTGCTG |
| Internal bar code: | AAACCTGCTTATATTGCGTAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1450 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 1789 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGGATTACCGGCTTGGT |
| Suggested primer 2: | CACAGCCTCCTTCACTGTCA |