| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213024 |
| Chromosome: | chromosome 3 |
| Location: | 3744235 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g169800 | (1 of 2) PTHR10060:SF22 - CELL DEATH-RELATED NUCLEASE 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCCTCCTGTGCAGCCCCACAGGCCCTGCC |
| Internal bar code: | ACGGATTACGCCTCTGAGCACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 403 |
| LEAP-Seq percent confirming: | 99.8037 |
| LEAP-Seq n confirming: | 1017 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACTCCTTTGCCAACACCAC |
| Suggested primer 2: | GTTACGGTCATGGCGAAAGT |