| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213024 |
| Chromosome: | chromosome 9 |
| Location: | 4263290 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394547 | (1 of 3) 2.4.1.19 - Cyclomaltodextrin glucanotransferase / Cyclodextrin glycosyltransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGTTGCCATGAGACTCTTCCTCAGTATG |
| Internal bar code: | TCGGCGCGGAGCTGCTAGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1079 |
| LEAP-Seq percent confirming: | 99.6971 |
| LEAP-Seq n confirming: | 5267 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGGCATGACGATGAAGAT |
| Suggested primer 2: | GCGTTGAGTACGCAAGATGA |