| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213051 |
| Chromosome: | chromosome 7 |
| Location: | 236772 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313800 | CGL138 | Conserved in the Green Lineage | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTGCATGGGCACGCTGCACAACGCATG |
| Internal bar code: | GCGGGTAGCTAAAATGGGCGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 274 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 397 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGACCGTTTATGTTCCGT |
| Suggested primer 2: | GGAGCAGCAGTAAAAGTGGC |