| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213095 |
| Chromosome: | chromosome 7 |
| Location: | 1396408 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322800 | CSA4 | Chlorophyceae-specific gene family A protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAATGGTGCCGGCAGCCAGTCGCGTCAGC |
| Internal bar code: | ATAAGGTGCAGGAGGCGTTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 379 |
| LEAP-Seq percent confirming: | 99.4947 |
| LEAP-Seq n confirming: | 4332 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCATTAAGCTGCTCCTCGGT |
| Suggested primer 2: | GAGCTGAACAAGGAGTTCCG |