| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213108 |
| Chromosome: | chromosome 1 |
| Location: | 6256687 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g044750 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACACGGAGGCAGAGTACCCAGGGAAGAAC |
| Internal bar code: | ACGTCGAGTGGGGGGCAAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 99.6575 |
| LEAP-Seq n confirming: | 873 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAAGTGCATGTGAGCCTGT |
| Suggested primer 2: | ACGATGACAAGGTCCGAATC |