Insertion junction: LMJ.RY0402.213129_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.213129
Chromosome:chromosome 15
Location:992438
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre15.g639000 OPR88,RAP22,CLR11 RAP-domain containing protein 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAACTGGATGCTCGCCAGTTTGCGAGCTGC
Internal bar code:TCAAACAACGCGAACCATTGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:879
LEAP-Seq percent confirming:99.5545
LEAP-Seq n confirming:31732
LEAP-Seq n nonconfirming:142
LEAP-Seq n unique pos:44

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCCAGATCCCAGAAGATG
Suggested primer 2:GAGTCAGCGCTCACATACCA