| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.213133 |
| Chromosome: | chromosome_5 |
| Location: | 2061298 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre05.g234550 | FBA3 | Fructose-1,6-bisphosphate aldolase | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTGACTCGATTGTACATTGGCGGTTGCGAT |
| Internal bar code: | GCGGCTTACTGGTTGGGGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1049 |
| LEAP-Seq percent confirming: | 99.4265 |
| LEAP-Seq n confirming: | 7628 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATAACCGCGTTCTTTCCCTT |
| Suggested primer 2: | GTTGCTGTGTGTGTGCTGTG |