| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.213153 |
| Chromosome: | chromosome 4 |
| Location: | 1182337 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215650 | (1 of 1) PF08743 - Nse4 C-terminal (Nse4_C) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGTCTCTCCCAGTCAGCTGCCATTCCAGC |
| Internal bar code: | AGATGCTCGAGCGACTAACCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 598 |
| LEAP-Seq percent confirming: | 99.3272 |
| LEAP-Seq n confirming: | 2805 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTGCAGTTCATCATGTCG |
| Suggested primer 2: | TGTCTGGTTAGCGACCTGTG |