| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.213160 |
| Chromosome: | chromosome 6 |
| Location: | 6558680 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g293450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGTGCCCTTCCTCACCCCCAATGGCCCA |
| Internal bar code: | GATAGCACTGCCCGCGGCCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 419 |
| LEAP-Seq percent confirming: | 96.6493 |
| LEAP-Seq n confirming: | 1298 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGTTCTCGTGTATGTGTG |
| Suggested primer 2: | CCAGCCCAAACTAAACCAAA |